Cell migration refers to the movement of cells after receiving a migration signal or feeling a concentration gradient of certain substances. During the movement, the cell repeats the cyclic process of extending the synapse / pseudopod forward and then pulling the rear soma. The cytoskeleton and its binding proteins, as well as the intercellular substance, are the material basis of this process, and there are many other substances that precisely regulate it. There are many types of cell movements, including physiological movements, such as cell movements during development, and changes in the position of germ cells and stem cells during maturation. There are also pathological changes, such as tumor migration and invasion.
From cancer to metastasis, blood vessel supply and division and proliferation have always been hot spots in medical and biological research. Cancer cell proliferation is out of control, and a large number of offspring can be reproduced in a short time. This will first cause cramped growth space and nutrients, such as oxygen tension. In this way, a necrotic area will form in the malignant tumor. As mentioned above in the tissue damage, the body will try to "repair" these damages. Necrotic tissue releases a series of pro-angiogenic factors, such as vascular endothelial growth factor, and various immune cells, such as macrophages. Macrophages also release large amounts of pro-angiogenic cytokines and growth factors. Therefore, tumor research is accompanied by complex cellular movements, such as tumor cells moving along the circulatory system, and vascular endothelial cells and immune cells entering tumor entities.
The scratch method is a classic method for detecting cell behavior. Make a mark on the tiled cell monolayer, and then wash and change the culture medium, the cells will migrate from the original position to the scratch. Changes in the width and area of the trace can be used to monitor the rate and ability of cell migration.
In the past, when doing scratch experiments, there were many restrictions: first, the holes in the microwell plate must not be too small, the smaller the holes, the more difficult it is to reach the tip; secondly, the edges of the traces are skewed and cannot form a straight line; The width of the scratches is also uneven. This caused a great deal of trouble for the scratch time gradient experiment. In many photographing processes, due to the difference in the width of the scratch, the resetting of the scratch photographing position is very demanding. However, with the occurrence of cell migration, the in situ morphology and distribution of cells also change dynamically. Therefore, resetting the photo position of the scratch has become a hard work: since it can't be found accurately, then all of them are taken; since the width of each position is different, then all of them are counted.
Scratch kit with MuviCyte ™ long-term live-cell imaging system. One swipe to solve all the problems.
With Scratcher's neat 96-pin, you can make uniform width scratches on the bottom surface of a 96-well microplate.
With MuviCyte ™ long-term live-cell imaging system, you can focus on a field of view without stopping. A jitter-free video is then generated.
With the help of professional scratch analysis software, analyze the scratch width, area, and healing speed. The parameters that can be obtained include:
Scratch healing speed
Relative scratch density
The original cell area, the original scratch area, the scratch boundary, and the area of the migrated cells are accurately divided to ensure the accuracy of the analysis results. Complete the entire experiment with ease, and never have to stay up late to take a scratch.
MuviCyte ™ has been launched on January 1, 2020. With its multiple fluorescence channels and multiple objective lens options, it can complete the shooting and observation of a variety of complex and complex cell models. It is available in many fields such as tumor immunity and stem cells Important applications.
Scan the QR code below to download PerkinElmer's MuviCyte ™ live cell imaging system.