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Your location : 幸运飞艇计划免费软件 > Download > Apolipoprotein ELISA Test Reagents
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Description of Apolipoprotein ELISA Detection Reagent

Last updated: 2019/8/1 17:09:54

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Detailed introduction:

Description of Apolipoprotein ELISA Detection Reagent

The summer night is beautiful, and the refreshing evening breeze hummed to Xiaoqu and brought us a hint of coolness. In the night sky, the stars blinked and quietly listened to Sister Moon's story. This beautiful story inspired the imagination of the stars. The stars were whispering and discussing. Is it talking about the speech and where to give a speech? There is a quiet surrounding, only the evening wind sings low, and the moonlight is flowing toward The never-ending stream sprinkles meteor flowers that bloom at night, as if everything is alive. The firefly held a small lantern and looked at the flowers attentively, letting them grow up and bloom. The following describes the description of apolipoprotein ELISA detection reagents.

The basic principle of the ELISA method is:

① The antigen or antibody is bound to the surface of a certain solid carrier, and its immune activity is maintained.

② The antigen or antibody is linked with an enzyme to form an enzyme-labeled antigen or antibody. This enzyme-labeled antigen or antibody retains both its immune activity and enzyme activity. During the assay, the test specimen (the antibody or antigen in the assay) and the enzyme-labeled antigen or antibody are reacted with the antigen or antibody on the surface of the solid phase carrier in different steps. The washing method is used to separate the antigen-antibody complex formed on the solid phase carrier from other substances, and the amount of the enzyme bound to the solid phase carrier after zui is proportional to the amount of the test substance in the specimen. After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so it can be qualitatively or quantitatively analyzed according to the depth of the color reaction. Due to the high catalytic frequency of the enzyme, the effect of the reaction can be greatly amplified, so that the method has a high sensitivity. Therefore, ELISA detection is a comprehensive technique of localization, qualitative and quantitative (sensitivity can reach ng ~ pg level per ml).

Apolipoprotein ELISA Test Kit

Steps:

2 孔、阳性对照2 孔、空白对照1孔(空白对照孔不加样品及酶标试剂,其余各步操作相同) 1. Numbering: Number the corresponding microwells in sequence. Each plate should be set with 2 wells for negative control, 2 wells for positive control, and 1 well for blank control.

2. Adding samples: Add 50 μl of negative control and positive control to the negative and positive control wells, respectively. Then add 40µl of the sample dilution to the well of the sample to be tested, and then add 10µl of the sample to be tested. Add the sample to the bottom of the well of the microtiter plate, try not to touch the wall of the well, and gently shake to mix.

3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes.

4. Dosing: Dilute 30 times concentrated washing solution with distilled water 30 times and set aside.

5. Washing: Carefully peel off the sealing plate film, discard the liquid, and dry it. Fill each well with washing liquid, leave it for 30 seconds and discard it. Repeat this 5 times and pat dry.

6. Add enzyme: Add 50µl of enzyme-labeled reagent to each well, except for blank wells.

7. Incubation: The operation is the same as 3.

8. Washing: The operation is the same as 5.

9. Color development: Add the color developer A50µl to each well, and then add the color developer B50µl, shake gently and mix, and develop the color at 37 ° C in the dark for 10 minutes.

10. Termination: Add 50 µl of stop solution to each well to stop the reaction (the blue color turns to yellow at this time).

11. Determination: Measure the absorbance (OD value) of each well sequentially with a blank air conditioner at a wavelength of 450nm. The measurement should be performed within 15 minutes after adding the stop solution.

After-sales service ?:

1. Free replacement if there is a quality problem, as stated on the contract. (Quality problems include improper shipping, customers cannot detect results during use, etc.)

2. Full technical guidance. (Including the collection of pre-sale specimens, places that are not understood during use, data analysis after the experiment, if there is any question, our technology will call you immediately)

3. Provide free testing services. (You just need to send the specimens, we will save you time, help you produce results, raw data, analysis data can be provided, the general time is about 5 days)

4. If the customer has any problem, we will give a solution within one working day. After sales and technology are dedicated to customer service.

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